Sorbitol dehydrogenases in Acetobacter suboxydans.

نویسندگان

J T CUMMINS

V H CHELDELIN

T E KING

چکیده

It has previously been shown that cell-free extracts of Acetobacter subozydans possess two pathways for the oxidation of sorbitol (1). In the presence of DPN,l fructose is produced; with TPN, sorbose is formed. The primary purpose of this paper is to describe the separation of these two dehydrogenases from each other. The DPN enzyme free from TPN activity has been concentrated about lBfold, whereas the TPN enzyme is fragile and apparently destroyed during fractionation.

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